1HH8
The active N-terminal region of p67phox: Structure at 1.8 Angstrom resolution and biochemical characterizations of the A128V mutant implicated in chronic granulomatous disease
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 110 |
Collection date | 2000-10-15 |
Spacegroup name | P 31 |
Unit cell lengths | 67.670, 67.670, 50.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.182 |
Rwork | 0.182 |
R-free | 0.20400 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 18.900 * |
Data reduction software | DENZO |
Data scaling software | CCP4 |
Phasing software | MLPHARE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.047 * | 0.198 * |
Total number of observations | 95808 * | |
Number of reflections | 23850 | |
<I/σ(I)> | 12.4 | 3.6 |
Completeness [%] | 99.5 | 99.5 |
Redundancy | 4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.5 | 20 * | 100 MM NA CITRATE PH 4.5, 17% PEG 2K MME, 10% GLYCEROL |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5-6 (mg/ml) | |
2 | 1 | reservoir | PEG2000 MME | 17 (%) | |
3 | 1 | reservoir | sodium citrate | 100 (mM) | |
4 | 1 | reservoir | glycerol | 10 (%) | |
5 | 1 | reservoir | dithiothreitol | 10 (mM) |