1HH8
The active N-terminal region of p67phox: Structure at 1.8 Angstrom resolution and biochemical characterizations of the A128V mutant implicated in chronic granulomatous disease
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 110 |
| Collection date | 2000-10-15 |
| Spacegroup name | P 31 |
| Unit cell lengths | 67.670, 67.670, 50.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| R-free | 0.20400 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 18.900 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 |
| Phasing software | MLPHARE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.047 * | 0.198 * |
| Total number of observations | 95808 * | |
| Number of reflections | 23850 | |
| <I/σ(I)> | 12.4 | 3.6 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.5 | 20 * | 100 MM NA CITRATE PH 4.5, 17% PEG 2K MME, 10% GLYCEROL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-6 (mg/ml) | |
| 2 | 1 | reservoir | PEG2000 MME | 17 (%) | |
| 3 | 1 | reservoir | sodium citrate | 100 (mM) | |
| 4 | 1 | reservoir | glycerol | 10 (%) | |
| 5 | 1 | reservoir | dithiothreitol | 10 (mM) |
