1HG3
Crystal structure of tetrameric TIM from Pyrococcus woesei.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Wavelength(s) | 0.9795,0.9796,0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.000, 89.100, 145.100 |
| Unit cell angles | 90.00, 92.80, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.700 |
| R-factor | 0.254 |
| Rwork | 0.254 |
| R-free | 0.28600 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.410 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.200 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.076 | 0.112 |
| Total number of observations | 386571 * | |
| Number of reflections | 25844 | |
| Completeness [%] | 90.1 | 89.7 |
| Redundancy | 15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4 * | Bell, G.S., (1998) Acta crystallog., D54, 1419. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 2-carboxyethylphosphonic acid | 20 (mM) | |
| 2 | 1 | reservoir | sodium acetate | 0.1 (M) | |
| 3 | 1 | reservoir | PEG4000 | 7 (%) | |
| 4 | 1 | drop | protein | 10 (mg/ml) |
