1HFS
CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF HUMAN FIBROBLAST STROMELYSIN-1 INHIBITED WITH THE N-CARBOXY-ALKYL INHIBITOR L-764,004
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1994-08-29 |
| Detector | RIGAKU |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 84.140, 85.350, 55.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.21700 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 24.000 * |
| Data reduction software | R-AXIS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Rmerge | 0.034 |
| Number of reflections | 21587 |
| Completeness [%] | 97.0 |
| Redundancy | 3.17 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 16.5 (mg/ml) | |
| 2 | 1 | drop | L-764,004 | 1.2 (M) | |
| 3 | 1 | drop | 5.0 (mM) | ||
| 4 | 1 | drop | 0.02 (%) | ||
| 5 | 1 | drop | MES | 20 (mM) | |
| 6 | 1 | reservoir | PEG6000 | 10 (%) | |
| 7 | 1 | reservoir | 5 (%sat) | ||
| 8 | 1 | reservoir | 0.02 (%) | ||
| 9 | 1 | reservoir | cacodylate | 0.1 (M) |
