1HFC

1.56 ANGSTROM STRUCTURE OF MATURE TRUNCATED HUMAN FIBROBLAST COLLAGENASE

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	109.210, 44.570, 36.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.560 *
R-factor	0.174
Rwork	0.174
RMSD bond length	0.020
RMSD bond angle	2.700
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	2.830 *
High resolution limit [Å]	1.560 *	1.560 *
Rmerge	0.064 *	0.269 *
Total number of observations	64262 *
Number of reflections	23912 *	3218 *
Completeness [%]	89.7 *	73.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	15 (mg/ml)
2	1	1	Tris-HCl	2.5 (mM)
3	1	1		5.0 (mM)
4	1	1		0.4 (M)
5	1	1		3 (mM)
6	1	1	hydroxamate inhibitor	1 (mM)