1HFB
Crystal structure of the tyrosine-regulated 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Saccharomyces cerevisiae complexed with phosphoenolpyruvate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-09-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 82.000, 93.800, 104.500 |
| Unit cell angles | 65.60, 85.60, 75.40 |
Refinement procedure
| Resolution | 40.000 - 1.900 |
| R-factor | 0.2079 |
| Rwork | 0.208 |
| R-free | 0.26100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CHAINS A AND B OF PDB ENTRY 1QR7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | |
| High resolution limit [Å] | 1.900 | 1.900 * |
| Rmerge | 0.037 | 0.354 * |
| Total number of observations | 423490 * | |
| Number of reflections | 210365 | |
| Completeness [%] | 96.3 | 89.3 * |
| Redundancy | 2.9 * | 1.2 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 9 | 21 * | pH 9.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG3000 | 14 (%) | |
| 2 | 1 | reservoir | dithiothreitol | 1 (mM) | pH9.0 |
| 3 | 1 | reservoir | Tris-HCl | 10 (mM) |
