1HDO
Human biliverdin IX beta reductase: NADP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, Hamburg |
Beamline | BW7B |
Temperature [K] | 100 |
Collection date | 1999-11-15 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.000, 49.200, 106.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.150 |
R-factor | 0.124 |
R-free | 0.15800 |
Structure solution method | SIRAS |
RMSD bond length | 0.015 |
RMSD bond angle | 0.032 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.700 | 1.210 |
High resolution limit [Å] | 1.150 | 1.150 |
Rmerge | 0.042 | 0.188 |
Total number of observations | 426976 * | |
Number of reflections | 75534 | |
<I/σ(I)> | 11 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6.5 | 30% PEG 3350, 0.2 M AMMONIUM SULFATE, 0.1 M SODIUM CACODYLATE PH 6.5, NADP TO ADDED TO A FINAL CONCENTRATION OF 2.5 MM |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6.7 (mg/ml) | |
2 | 1 | drop | NADP | 2.5 (mM) | |
3 | 1 | drop | PEG8000 | 5 (%(w/v)) | |
4 | 1 | drop | ammonium sulfate | 0.03 (M) | |
5 | 1 | drop | sodium cacodylate | 0.02 (M) |