1HDC

MECHANISM OF INHIBITION OF 3ALPHA,20BETA-HYDROXYSTEROID DEHYDROGENASE BY A LICORICE-DERIVED STEROIDAL INHIBITOR

Experimental procedure

Spacegroup name	P 1
Unit cell lengths	72.050, 60.040, 59.840
Unit cell angles	101.78, 104.41, 96.31

Refinement procedure

Resolution	8.000 * - 2.200
Rwork	0.194
RMSD bond length	0.012
RMSD bond angle	1.800

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.080 *
High resolution limit [Å]	2.200 *	2.200 *
Total number of observations	48972 *
Number of reflections	34405 *
Completeness [%]	71.0 *	65 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.3 *		referred to J.Biol.Chem. 261.1306-1308 1986 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	phosphate	1.40-1.60 (M)
2	1	drop	protein	13-15 (mg/ml)
3	1	drop	phosphate	0.7 (M)