1HC7

Prolyl-tRNA synthetase from Thermus thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-04-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	132.300, 191.100, 125.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.430
R-factor	0.206
Rwork	0.206
R-free	0.23400
Structure solution method	SIRAS
RMSD bond length	0.006
RMSD bond angle	1.286
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	RSPS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.490
High resolution limit [Å]	2.430	2.430
Rmerge	0.053	0.244
Number of reflections	117904
<I/σ(I)>		2.9
Completeness [%]	98.6	93.1
Redundancy	3.8	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.9		SEE REFERENCE 2, pH 7.90