1HAG

THE ISOMORPHOUS STRUCTURES OF PRETHROMBIN2, HIRUGEN-AND PPACK-THROMBIN: CHANGES ACCOMPANYING ACTIVATION AND EXOSITE BINDING TO THROMBIN

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	72.850, 71.320, 72.630
Unit cell angles	90.00, 101.10, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.169
Rwork	0.169
RMSD bond length	0.022
RMSD bond angle	0.053 *
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	2.000 *	2.000 *
Rmerge	0.056 *
Total number of observations	23973 *
Number of reflections	16642 *
Completeness [%]	66.0 *	43 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.3 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium phosphate	0.1 (M)
2	1	reservoir	PEG8000	27 (%)
3	1	reservoir		1 (mM)