1HA4
GammaS crystallin C terminal domain from Homo Sapiens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Collection date | 2000-03-18 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 61.367, 61.367, 241.665 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 100.000 - 2.400 |
| R-factor | 0.2159 |
| Rwork | 0.216 |
| R-free | 0.26390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a7h |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.388 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.700 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.057 * | 0.107 * |
| Total number of observations | 71559 * | |
| Number of reflections | 10955 | 809 * |
| <I/σ(I)> | 23.8 | 5.7 |
| Completeness [%] | 97.2 | 99.4 |
| Redundancy | 6.5 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 0.1M SODIUM ACETATE, 0.2M AMMONIUM SULPHATE, 24% PEG MONOMETHYLETHER, pH 5.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Bis-Tris propane-HCl | 25 (mM) | pH7.5 |
| 3 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 4 | 1 | reservoir | sodium acetate | 0.1 (M) | pH5.0 |
| 5 | 1 | reservoir | PEG2000MME | 24 (%) |
