1H9O
PHOSPHATIDYLINOSITOL 3-KINASE, P85-ALPHA SUBUNIT: C-TERMINAL SH2 DOMAIN COMPLEXED WITH A TYR751 PHOSPHOPEPTIDE FROM THE PDGF RECEPTOR, CRYSTAL STRUCTURE AT 1.79 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-09-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 59.000, 32.800, 54.900 |
| Unit cell angles | 90.00, 96.20, 90.00 |
Refinement procedure
| Resolution | 54.000 - 1.790 |
| R-factor | 0.168 |
| Rwork | 0.163 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pic |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.000 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 54.000 |
| High resolution limit [Å] | 1.790 |
| Rmerge | 0.050 |
| Total number of observations | 36576 * |
| Number of reflections | 9283 |
| Completeness [%] | 93.0 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 40MG/ML PROTEIN IN 20 MM PHOSPHATE PH6.2 + PEG8000, RESERVOIR WITH SAME PHOSPHATE + PEG8000 + 250MM NACL, pH 7.00 |
