1H7N
SCHIFF-BASE COMPLEX OF YEAST 5-AMINOLAEVULINIC ACID DEHYDRATASE WITH LAEVULINIC ACID AT 1.6 A RESOLUTION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-06-15 |
Detector | MARRESEARCH |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 103.200, 103.200, 167.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 13.100 - 1.600 |
R-factor | 0.217 |
R-free | 0.26500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ylv |
RMSD bond length | 0.009 |
RMSD bond angle | 0.025 |
Data reduction software | MOSFLM |
Data scaling software | Agrovata |
Phasing software | CCP4 |
Refinement software | SHELX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 13.100 | 1.650 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.061 | 0.227 |
Number of reflections | 59510 | |
<I/σ(I)> | 6.1 | 3 |
Completeness [%] | 99.6 | 99.6 |
Redundancy | 5.3 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 8 | Erskine, P.T., (1997) Protein Sci., 6, 1774. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 2 (mg/ml) | |
2 | 1 | reservoir | Tris-HCl | 0.2 (M) | |
3 | 1 | reservoir | PEG6000 | 50 (%(w/v)) | 0.040ml-0.240ml |
4 | 1 | reservoir | zinc sulfate | 4mg | |
5 | 1 | reservoir | beta-mercaptoethanol | 0.100ml in 10ml water |