1H74
CRYSTAL STRUCTURE OF HOMOSERINE KINASE COMPLEXED WITH ILE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.993, 128.721, 109.423 |
| Unit cell angles | 90.00, 105.57, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fwl |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.620 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.072 |
| Number of reflections | 131766 |
| <I/σ(I)> | 26.9 |
| Completeness [%] | 99.3 |
| Redundancy | 4.05 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | PEG4000, SODIUM ACETATE, pH 8.00 |
