1H6R
The oxidized state of a redox sensitive variant of green fluorescent protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-02-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.864, 93.915, 140.551 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 78.090 - 1.500 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yfp |
| RMSD bond length | 0.011 |
| RMSD bond angle | 27.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.090 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.033 | 0.439 |
| Total number of observations | 750960 * | |
| Number of reflections | 116321 | |
| <I/σ(I)> | 18.3 | 2 |
| Completeness [%] | 89.1 | 72.9 |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | 22 * | 100 MM HEPES, PH 8.0, 100 MM MGCL2, AND 14% PEG4000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 24 (mg/ml) | |
| 2 | 1 | drop | HEPES | 10 (mM) | pH8.0 |
| 3 | 1 | reservoir | HEPES | 100 (mM) | pH8.0 |
| 4 | 1 | reservoir | 100 (mM) | ||
| 5 | 1 | reservoir | PEG4000 | 14 (%(w/v)) |
