1H6E

MU2 ADAPTIN SUBUNIT (AP50) OF AP2 ADAPTOR (SECOND DOMAIN), COMPLEXED WITH CTLA-4 INTERNALIZATION PEPTIDE TTGVYVKMPPT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Collection date	1999-04-26
Detector	ADSC CCD
Spacegroup name	P 64
Unit cell lengths	126.779, 126.779, 74.161
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 * - 3.600
Rwork	0.278
R-free	0.31700
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1bxx
RMSD bond length	0.008
RMSD bond angle	25.700 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CCP4
Refinement software	CNX (2000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.200	3.800
High resolution limit [Å]	3.600	3.600
Rmerge	0.133	0.354
Total number of observations	14633 *
Number of reflections	6681	992 *
<I/σ(I)>	4	1.7
Completeness [%]	83.2	85.9
Redundancy	2.2	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5 *	15 *	HANGING DROPS CONTAINING A 1:1 MIXTURE OF COMPLEX SOLUTION 6MG/ML PROTEIN AND 1MM 11MER PEPTIDE SOLUTION IN 20MM HEPES PH7.5, 1MM DTT AND RESERVOIR BUFFER 1.4-2.4M SODIUM CHLORIDE, 0.1M MES PH6.2-7.0, 0.4M NA/K PHOSPHATE, 15% GLYCEROL, pH 7.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	drop	peptide	1 (mM)
3	1	drop	HEPES	20 (mM)	pH7.5
4	1	drop	dithiothreitol	1 (mM)
5	1	reservoir	MES	0.1 (M)	pH6.2-7.0
6	1	reservoir		1.4-2.4 (M)
7	1	reservoir	sodium potassium phosphate	0.4 (M)
8	1	reservoir	glycerol	15 (%(v/v))