1H5T
Thymidylyltransferase complexed with Thymidylyldiphosphate-glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Collection date | 2000-06-15 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.810, 119.490, 81.170 |
| Unit cell angles | 90.00, 112.67, 90.00 |
Refinement procedure
| Resolution | 12.000 - 1.900 |
| Rwork | 0.174 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.048 |
| Number of reflections | 98078 |
| <I/σ(I)> | 14.7 |
| Completeness [%] | 97.5 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | pH 5.50 |
