1H4O
Monoclinic form of human peroxiredoxin 5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-05-15 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 130.790, 66.490, 141.240 |
| Unit cell angles | 90.00, 117.54, 90.00 |
Refinement procedure
| Resolution | 24.000 - 1.950 |
| R-factor | 0.166 |
| Rwork | 0.165 |
| R-free | 0.19000 |
| Structure solution method | SIR |
| RMSD bond length | 0.018 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.000 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.032 | 0.142 |
| Number of reflections | 151926 | |
| <I/σ(I)> | 21 | 5 |
| Completeness [%] | 96.6 | 96.6 |
| Redundancy | 1.83 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.3 | PROTEIN WAS CRYSTALLIZED FROM 1.6 M AMMONIUM SULFATE, 0.1 M SODIUM CITRATE BUFFER PH 5.3, 0.2 M POTASSIUM SODIUM TARTRATE, 1 MM DTT, 0.02 % (W/V) SODIUM AZIDE |
