1H4L

Structure and regulation of the CDK5-p25(nck5a) complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-05-15
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	149.090, 89.760, 82.570
Unit cell angles	90.00, 93.08, 90.00

Refinement procedure

Resolution	20.000 * - 2.650
R-factor	0.236
Rwork	0.236
R-free	0.28400 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fin
RMSD bond length	0.021 *
RMSD bond angle	2.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.740
High resolution limit [Å]	2.650	2.650
Rmerge	0.043	0.233
Total number of observations	67318 *
Number of reflections	32449
<I/σ(I)>	16	3.6
Completeness [%]	93.9 *	78.5
Redundancy	2.26

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.6	20 *	used microseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	20 (%)
2	1	reservoir		200 (mM)
3	1	drop	protein	7 (mg/ml)