1H3N
Leucyl-tRNA synthetase from Thermus thermophilus complexed with a sulphamoyl analogue of leucyl-adenylate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-06-15 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 102.410, 155.590, 176.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.000 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.22900 * |
| Structure solution method | MAD |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.300 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.060 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.077 | 0.390 |
| Number of reflections | 88328 | |
| <I/σ(I)> | 7.4 | 2 |
| Completeness [%] | 90.4 | 60.2 |
| Redundancy | 4.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | Yaremchuk, A., (2000) Acta Crystallogr.,Sect.D, 56, 667. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Bis-Tris propane | 50 (mM) | pH6.0-6.5 |
| 3 | 1 | drop | dithiothreitol | 2 (mM) | |
| 4 | 1 | drop | 20 (mM) | ||
| 5 | 1 | drop | 1 (mM) | ||
| 7 | 1 | drop | ammonium sulfate | 15 (%sat) | |
| 8 | 1 | reservoir | ammonium sulfate | 30 (%) |
