1H21
A novel iron centre in the split-Soret cytochrome c from Desulfovibrio desulfuricans ATCC 27774
Replaces: 1DDCExperimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-02-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 96.060, 100.130, 109.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.26400 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 24.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.038 | 0.074 |
| Number of reflections | 34228 | |
| <I/σ(I)> | 9.7 | 9.7 |
| Completeness [%] | 93.0 | 86 |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 5 | Matias, P.M., (1997) J.Biol.Inorg.Chem., 2, 507. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | PEG8000 | 17-19 (%(w/v)) | |
| 3 | 1 | drop | sodium acetate | 0.1 (M) | pH5.0 |
| 4 | 1 | reservoir | agarose | 0.1-0.2 (%(w/v)) | |
| 5 | 1 | reservoir | PEG8000 | 12-15 (%) | |
| 6 | 1 | reservoir | sodium acetate | 0.1 (M) | pH5.0 |
