1H21
A novel iron centre in the split-Soret cytochrome c from Desulfovibrio desulfuricans ATCC 27774
Replaces: 1DDCExperimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1996-02-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 96.060, 100.130, 109.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.199 |
Rwork | 0.199 |
R-free | 0.26400 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 24.500 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.560 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.038 | 0.074 |
Number of reflections | 34228 | |
<I/σ(I)> | 9.7 | 9.7 |
Completeness [%] | 93.0 | 86 |
Redundancy | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 5 | Matias, P.M., (1997) J.Biol.Inorg.Chem., 2, 507. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | drop | PEG8000 | 17-19 (%(w/v)) | |
3 | 1 | drop | sodium acetate | 0.1 (M) | pH5.0 |
4 | 1 | reservoir | agarose | 0.1-0.2 (%(w/v)) | |
5 | 1 | reservoir | PEG8000 | 12-15 (%) | |
6 | 1 | reservoir | sodium acetate | 0.1 (M) | pH5.0 |