1GZ5

Trehalose-6-phosphate synthase. OtsA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2001-11-15
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	103.612, 125.400, 176.607
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.000 - 2.430
R-factor	0.207
Rwork	0.206
R-free	0.24000 *
Structure solution method	MAD
RMSD bond length	0.019
RMSD bond angle	1.950 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.540
High resolution limit [Å]	2.450	2.450
Rmerge	0.056	0.430 *
Number of reflections	86205
<I/σ(I)>	17	2.4
Completeness [%]	99.9	99.5
Redundancy	2.8	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5		14% PEG4000, 0.15M NAAC, 0.1M IMIDAZOLE PH 7.5, 10MM MG-UDP, 10MM G6P

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG4000	14 (%(v/v))
2	1	1		0.15 (M)
3	1	1	imidazole	0.1 (M)	pH7.5
4	1	1		200 (mM)