1GZ3
Molecular mechanism for the regulation of human mitochondrial NAD(P)+-dependent malic enzyme by ATP and fumarate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Collection date | 2001-02-15 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.815, 192.653, 110.161 |
| Unit cell angles | 90.00, 89.77, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1DO8_D |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | COMO |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.050 | 0.087 |
| Number of reflections | 250429 | |
| <I/σ(I)> | 18 | 11 |
| Completeness [%] | 91.0 | 78 |
| Redundancy | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 9% PEG20K, PH 5.5 NA CITRATE, 5% MPD |
