1GXD
proMMP-2/TIMP-2 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-05-15 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 75.696, 374.575, 191.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 12.940 * | |
High resolution limit [Å] | 3.100 | 3.100 * |
Rmerge | 0.110 * | |
Total number of observations | 475848 * | |
Number of reflections | 46264 | |
<I/σ(I)> | 10 | |
Completeness [%] | 94.1 | 70.8 * |
Redundancy | 10.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.25 * | 4 * | pH 8.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 11 (mg/ml) | |
2 | 1 | reservoir | 0.2 (M) | ||
3 | 1 | reservoir | reduced | 0.2 (mM) | |
4 | 1 | reservoir | oxidized glutathione | 0.2 (mM) | |
5 | 1 | reservoir | PEG4000 | 4 (%) | |
6 | 1 | reservoir | ammonium acetate | 0.1 (M) | pH6.25 |