1GX5

Hepatitis C Virus RNA Polymerase in Complex with GTP and Manganese

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-09-15
Detector	ADSC CCD
Spacegroup name	P 21 21 2
Unit cell lengths	67.076, 96.225, 95.995
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.780 - 1.700
R-factor	0.193
Rwork	0.193
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1csj
RMSD bond length	0.010
RMSD bond angle	22.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.740
High resolution limit [Å]	1.700	1.700
Rmerge	0.069	0.423
Number of reflections	68538
<I/σ(I)>	12.3	2.9
Completeness [%]	99.2	98.5 *
Redundancy	3.2	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	4 *	Bressanelli, S., (1999) Proc. Natl. Acad. Sci. U.S.A., 96, 13034. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	14 (mg/ml)
2	1	drop	HEPES	20 (mM)
3	1	drop	dithiothreitol	20 (mM)
4	1	drop	EDTA	1 (mM)
5	1	reservoir	PEG8000	5 (%(w/v))
6	1	reservoir	sodium citrate	0.1 (M)
7	1	reservoir	2-propanol	5 (%(v/v))