1GWH
Atomic resolution structure of Micrococcus Lysodeikticus catalase complexed with NADPH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X31 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 1994-09-15 |
Detector | RAXIS |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 106.700, 106.700, 106.250 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.000 * - 1.750* |
R-factor | 0.1113 * |
Rwork | 0.111 |
R-free | 0.13830 * |
Structure solution method | OTHER |
RMSD bond length | 0.014 |
RMSD bond angle | 1.600 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.1.11) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.000 * | |
High resolution limit [Å] | 1.750 * | 1.750 * |
Rmerge | 0.073 * | 0.095 * |
Number of reflections | 54476 * | |
<I/σ(I)> | 24.4 | |
Completeness [%] | 87.7 * | 43.7 * |
Redundancy | 5.7 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.2 | pH 5.20 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20-30 (mg/ml) | |
2 | 1 | drop | ammonium sulfate | 0.6-0.8 (M) | |
3 | 1 | drop | sodium acetate | 0.05 (M) | pH5.2 |
4 | 1 | reservoir | ammonium sulfate | 1.2-1.4 (M) |