1GW6
STRUCTURE OF LEUKOTRIENE A4 HYDROLASE D375N MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-09-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.430, 87.060, 99.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.950 - 2.200 |
| R-factor | 0.174 |
| Rwork | 0.174 |
| R-free | 0.22040 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hs6 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.510 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.083 | 0.206 |
| Number of reflections | 29456 | |
| <I/σ(I)> | 8.4 | 3.9 |
| Completeness [%] | 97.2 | 95.1 |
| Redundancy | 3.4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | liquid-liquid diffusion * | 6.8 | PEG8000, IMIDAZOLE, SODIUM ACETATE, YTTERBIUM CHLORIDE, BESTATIN, pH 6.80 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG8000 | 28 (%(v/v)) | |
| 2 | 1 | 1 | sodium acetate | 0.1 (mM) | |
| 3 | 1 | 1 | imidazole | 0.1 (mM) | pH6.8 |
| 4 | 1 | 1 | 5 (mM) | ||
| 5 | 1 | 2 | protein | 5 (mg/ml) | |
| 6 | 1 | 2 | Tris-HCl | 10 (mM) | pH8. |
