1GVH
The X-ray structure of ferric Escherichia coli flavohemoglobin reveals an unespected geometry of the distal heme pocket
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Spacegroup name | P 6 2 2 |
Unit cell lengths | 164.860, 164.860, 53.460 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 2.190 |
R-factor | 0.187 |
Rwork | 0.187 |
R-free | 0.24700 |
Structure solution method | MIR |
RMSD bond length | 0.008 |
RMSD bond angle | 1.801 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.000 |
High resolution limit [Å] | 2.190 |
Rmerge | 0.090 |
Number of reflections | 22448 |
Completeness [%] | 99.5 |
Redundancy | 11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 5.1 | 25 * | SODIUM ACETATE 0.1 M PH 5.1, PEG 3350 21-26%, NACL 0.2 M |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 22 (mg/ml) | |
2 | 1 | reservoir | sodium acetate | 0.1 (M) | pH5.1-5.3 |
3 | 1 | reservoir | PEG3350 | 21-26 (%) | |
4 | 1 | reservoir | 0.2 (M) |