1GV4

Murine apoptosis-inducing factor (AIF)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2000-06-15
Spacegroup name	P 21 21 21
Unit cell lengths	86.270, 109.914, 114.609
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.000
R-factor	0.216 *
Rwork	0.216
R-free	0.25700 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1d7y
RMSD bond length	0.020 *
RMSD bond angle	1.770 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.110	0.273 *
Number of reflections	62596
<I/σ(I)>	4.4	2.5
Completeness [%]	96.5	92.6 *
Redundancy	2.9	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.75		18% PEG MONOMETHYL ETHER 5000, 100 MM MGCL2, 50 MM HEPES PH, pH 7.75

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG5000	18 (%(w/v))
2	1	reservoir		80 (mM)
3	1	reservoir	HEPES	50 (mM)	pH7.75