1GU7
Enoyl thioester reductase from Candida tropicalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.328, 100.513, 80.686 |
| Unit cell angles | 90.00, 101.10, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 1.700 |
| R-factor | 0.174 |
| Rwork | 0.174 |
| R-free | 0.19200 |
| Structure solution method | MIR |
| RMSD bond length | 0.011 * |
| RMSD bond angle | 24.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.036 | 0.330 |
| Number of reflections | 113812 | 10941 * |
| <I/σ(I)> | 32.7 | 3.5 |
| Completeness [%] | 99.5 | 96.7 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | pH 6.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5.7 (mg/ml) | |
| 2 | 1 | drop | sodium phosphate | 50 (mM) | pH7.0 |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 5 | 1 | reservoir | ADA/NaOH | 0.1 (M) | pH6.5 |
| 6 | 1 | reservoir | 0.1 (M) |
