1GSY

GLUTATHIONE S-TRANSFERASE YFYF, CLASS PI, COMPLEXED WITH GLUTATHIONE

Experimental procedure

Source type	ROTATING ANODE
Source details	ELLIOTT GX-21
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	142.680, 162.570, 63.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.440
R-factor	0.208 *
Rwork	0.194
R-free	0.28700 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1glp
RMSD bond length	0.010
RMSD bond angle	2.020 *
Data reduction software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.400	2.440 *
Rmerge	0.087 *
Total number of observations	171889 *
Number of reflections	20740
Completeness [%]	82.1 *	50.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10.8 (mM)
2	1	drop	MES	50 (mM)
3	1	drop	PEG6000	8 (%)
4	1	reservoir	sodium potassium phospahte	0.3 (M)