1GR2
STRUCTURE OF A GLUTAMATE RECEPTOR LIGAND BINDING CORE (GLUR2) COMPLEXED WITH KAINATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-05-01 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.220, 63.070, 46.320 |
| Unit cell angles | 90.00, 92.99, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.900 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.27300 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.483 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 19209 |
| Completeness [%] | 97.2 |
| Redundancy | 3.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.6 * | Chen, G.-Q., (1998) Protein Sci., 7, 2623. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 25 (%) | |
| 2 | 1 | reservoir | 0.1 (M) | pH4.6 | |
| 3 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 4 | 1 | drop | protein | 7.5 (mg/ml) |
