1GQT
Activation of Ribokinase by Monovalent Cations
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-02-26 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.453, 62.767, 339.277 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.000 - 2.340 |
| R-factor | 0.207 * |
| Rwork | 0.193 |
| R-free | 0.27100 * |
| Structure solution method | OTHER |
| RMSD bond length | 0.017 * |
| RMSD bond angle | 1.720 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.000 | 2.380 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.091 | 0.377 |
| Total number of observations | 245133 * | |
| Number of reflections | 50296 | |
| <I/σ(I)> | 10.9 | 3.8 |
| Completeness [%] | 98.2 | 99.9 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.8 | 4 * | CRYSTALS WERE GROWN IN DROPS CONTAINING 0.3 MM WILD-TYPE RK, 5 MM D-RIBOSE, 10 MM AMP-PCP, 75 MM MGCL2, 60 MM CSCL, 10% 2-METHYL-2,4-PENTANEDIOL, 10% POLYETHYLENE GLYCOL 4000 AND 50 MM SODIUM ACETATE, PH 4.8. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RK | 0.3 (mM) | |
| 2 | 1 | drop | D-ribose | 5 (mM) | |
| 3 | 1 | drop | AMP-PCP | 10 (mM) | |
| 4 | 1 | drop | 75 (mM) | ||
| 5 | 1 | drop | 60 (mM) | ||
| 6 | 1 | drop | sodium acetate | 50 (mM) | pH4.8 |
| 7 | 1 | drop | MPD | 10 (%(w/v)) | |
| 8 | 1 | drop | PEG4000 | 10 (%(w/v)) |
