1GQT

Activation of Ribokinase by Monovalent Cations

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2000-02-26
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	55.453, 62.767, 339.277
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.000 - 2.340
R-factor	0.207 *
Rwork	0.193
R-free	0.27100 *
Structure solution method	OTHER
RMSD bond length	0.017 *
RMSD bond angle	1.720 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.000	2.380
High resolution limit [Å]	2.340	2.340
Rmerge	0.091	0.377
Total number of observations	245133 *
Number of reflections	50296
<I/σ(I)>	10.9	3.8
Completeness [%]	98.2	99.9
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.8	4 *	CRYSTALS WERE GROWN IN DROPS CONTAINING 0.3 MM WILD-TYPE RK, 5 MM D-RIBOSE, 10 MM AMP-PCP, 75 MM MGCL2, 60 MM CSCL, 10% 2-METHYL-2,4-PENTANEDIOL, 10% POLYETHYLENE GLYCOL 4000 AND 50 MM SODIUM ACETATE, PH 4.8.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	RK	0.3 (mM)
2	1	drop	D-ribose	5 (mM)
3	1	drop	AMP-PCP	10 (mM)
4	1	drop		75 (mM)
5	1	drop		60 (mM)
6	1	drop	sodium acetate	50 (mM)	pH4.8
7	1	drop	MPD	10 (%(w/v))
8	1	drop	PEG4000	10 (%(w/v))