1GQ2
Malic Enzyme from Pigeon Liver
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 277 |
| Collection date | 2001-02-15 |
| Detector | ADSC Q-4 |
| Spacegroup name | P 1 |
| Unit cell lengths | 124.149, 140.863, 167.079 |
| Unit cell angles | 90.05, 87.16, 75.63 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| R-factor | 0.211 * |
| Rwork | 0.210 |
| R-free | 0.25800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB |
| RMSD bond length | 0.008 |
| RMSD bond angle | 22.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | COMO |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.082 | 0.214 |
| Total number of observations | 570674 * | |
| Number of reflections | 314771 * | |
| <I/σ(I)> | 10.6 | 3.6 |
| Completeness [%] | 83.0 * | 76 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.4 * | 4 * | 100 MM SODIUM CITRATE, PH 5.5, 8% PEG6000, 1M LICL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | pigeon ME | 10 (mg/ml) | |
| 2 | 1 | drop | Tris | 20 (mM) | pH7.4 |
| 3 | 1 | drop | oxalate | 10 (mM) | |
| 4 | 1 | drop | 5 (mM) | ||
| 5 | 1 | drop | NADP+ | 0.23 (mM) | |
| 6 | 1 | drop | dithiothreitol | 2 (mM) | |
| 7 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.5 |
| 8 | 1 | reservoir | PEG6000 | 8 (%) | |
| 9 | 1 | reservoir | 1 (M) |
