1GPU
Transketolase complex with reaction intermediate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 110 |
| Collection date | 2001-04-15 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.300, 113.200, 159.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.860 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1trk |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.279 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.890 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.052 | 0.289 |
| Total number of observations | 333802 * | |
| Number of reflections | 104364 | |
| <I/σ(I)> | 15.9 | 3.5 |
| Completeness [%] | 92.9 | 89 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.9 | 4 * | pH 7.90 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG6000 | 13-17 (%(w/v)) | |
| 2 | 1 | reservoir | ThDP | 5 (mM) | |
| 3 | 1 | reservoir | 5 (mM) |
