1GPL

RP2 LIPASE

Experimental procedure

Detector technology	IMAGE PLATE
Detector	MAR scanner 180 mm plate
Spacegroup name	C 1 2 1
Unit cell lengths	62.000, 55.900, 144.000
Unit cell angles	90.00, 93.20, 90.00

Refinement procedure

Resolution	6.000 - 2.100 *
R-factor	0.188
Rwork	0.188
R-free	0.24600
RMSD bond length	0.013
RMSD bond angle	1.670
Data reduction software	DENZO
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.100 *	2.100 *
Rmerge	0.099 *
Number of reflections	27079
Completeness [%]	92.6	88.9 *
Redundancy	3.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *	20 *	used to seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	11 (mg/ml)
2	1	drop		50 (mM)
3	1	drop	MES	10 (mM)
4	1	reservoir	bicine	100 (mM)
5	1	reservoir		1 (M)
6	1	reservoir	PEG6000	10-15 (%(w/v))