1GPH

STRUCTURE OF THE ALLOSTERIC REGULATORY ENZYME OF PURINE BIOSYNTHESIS

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	158.800, 75.700, 94.100
Unit cell angles	90.00, 91.40, 90.00

Refinement procedure

Resolution	7.000 - 3.000
R-factor	0.182
Rwork	0.182
RMSD bond length	0.015
RMSD bond angle	3.300
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	3.000 *
Number of reflections	41267 *
Completeness [%]	90.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	7.9 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	7-8 (mg/ml)
10	1	2	PEG8000	15 (%)
11	1	2	Tris-HCl	50 (mM)
2	1	1	Tris-HCl	50 (mM)
3	1	1		3-4 (mM)
4	1	1	DTE	5 (mM)
5	1	1	AMP	2 (mM)
6	1	1	PEG8000	12-13 (%(w/v))
7	1	2		10 (mM)
8	1	2	DTE	5 (mM)
9	1	2	AMP	2 (mM)