1GPE
GLUCOSE OXIDASE FROM PENICILLIUM AMAGASAKIENSE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 290 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.600, 132.100, 151.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.164 |
Rwork | 0.160 |
R-free | 0.19800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gal |
RMSD bond length | 0.008 |
RMSD bond angle | 0.028 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MERLOT |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.880 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.068 | 0.132 |
Number of reflections | 101999 | |
<I/σ(I)> | 18.4 | 8.5 |
Completeness [%] | 94.7 | 90 |
Redundancy | 2.5 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.4 | Kalisz, H.M., (1990) J.Mol.Biol., 213, 207. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | glucose oxidase | 20 (mg/ml) | |
2 | 1 | drop | acetate | 50 (mM) | |
3 | 1 | drop | ammonium sulfate | 1.6 (M) | |
4 | 1 | reservoir | ammonium sulfate | 1.6 (M) | |
5 | 1 | drop | octanetriol solution | saturated |