1GOX

REFINED STRUCTURE OF SPINACH GLYCOLATE OXIDASE AT 2 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	I 4 2 2
Unit cell lengths	148.100, 148.100, 135.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.500 * - 2.000
R-factor	0.189
RMSD bond length	0.015
RMSD bond angle	0.043
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.000 *
Completeness [%]	70.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	8.3 *		referred to 'Lindqvist, Y.', (1979) J.Biol.Chem., 254, 7403-7404 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	20 (mg/ml)
2	1	1	Tris-HCl	0.05 (M)
3	1	2	tertiary butanol	5 (%(v/v))
4	1	2	FMN	0.25 (mg/ml)
5	1	2	Tris-HCl	0.05 (M)