1GLM

REFINED CRYSTAL STRUCTURES OF GLUCOAMYLASE FROM ASPERGILLUS AWAMORI VAR. X100

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	116.800, 104.400, 48.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.400
R-factor	0.122
RMSD bond length	0.012
RMSD bond angle	0.028
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.400 *
Rmerge	0.035 *
Total number of observations	57478 *
Number of reflections	20905 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.95 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	potassium phosphate	50 (mM)
3	1	drop	PEG6000	13 (%(w/v))
4	1	reservoir	phosphate	50 (mM)
5	1	reservoir	PEG6000	30 (%(w/v))