1GLA
STRUCTURE OF THE REGULATORY COMPLEX OF ESCHERICHIA COLI IIIGLC WITH GLYCEROL KINASE
Experimental procedure
Spacegroup name | I 2 2 2 |
Unit cell lengths | 123.400, 124.300, 133.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 5.000 * - 2.600 |
R-factor | 0.191 |
Rwork | 0.191 |
RMSD bond length | 0.013 |
RMSD bond angle | 3.100 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
High resolution limit [Å] | 2.600 * | 2.500 * |
Rmerge | 0.056 * | 0.232 * |
Total number of observations | 81994 * | |
Number of reflections | 24169 * | |
Completeness [%] | 76.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | sodium acetate | 0.5-0.8 (M) | |
3 | 1 | drop | MES | 100 (mM) | |
4 | 1 | reservoir | sodium acetate | 0.5-0.8 (M) | |
5 | 1 | reservoir | MES | 100 (mM) |