1GLA

STRUCTURE OF THE REGULATORY COMPLEX OF ESCHERICHIA COLI IIIGLC WITH GLYCEROL KINASE

Experimental procedure

Spacegroup name	I 2 2 2
Unit cell lengths	123.400, 124.300, 133.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.000 * - 2.600
R-factor	0.191
Rwork	0.191
RMSD bond length	0.013
RMSD bond angle	3.100
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.600 *	2.500 *
Rmerge	0.056 *	0.232 *
Total number of observations	81994 *
Number of reflections	24169 *
Completeness [%]	76.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	sodium acetate	0.5-0.8 (M)
3	1	drop	MES	100 (mM)
4	1	reservoir	sodium acetate	0.5-0.8 (M)
5	1	reservoir	MES	100 (mM)