1GIJ
HUMAN CYCLIN DEPENDENT KINASE 2 COMPLEXED WITH THE CDK4 INHIBITOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 294 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-07-07 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.020, 72.069, 53.982 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.200 |
| R-factor | 0.228 * |
| Rwork | 0.228 |
| R-free | 0.28600 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.946 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (98.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 * | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.060 | 0.171 |
| Total number of observations | 58086 * | |
| Number of reflections | 14195 | |
| <I/σ(I)> | 17.6 | |
| Completeness [%] | 92.3 | 87.3 |
| Redundancy | 4.1 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.2 | 4 * | HEPES, sodium chloride, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | HEPES-NaOH | 10 (mM) | pH7.4 |
| 3 | 1 | drop | 15 (mM) | ||
| 4 | 1 | reservoir | HEPES-NaOH | 800 (mM) | pH7.4 |
