1GH7

CRYSTAL STRUCTURE OF THE COMPLETE EXTRACELLULAR DOMAIN OF THE BETA-COMMON RECEPTOR OF IL-3, IL-5, AND GM-CSF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-07-18
Detector	MAR scanner 345 mm plate
Spacegroup name	H 3
Unit cell lengths	185.700, 185.700, 103.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.000 - 3.000
R-factor	0.267 *
Rwork	0.267
R-free	0.30400 *
RMSD bond length	0.009
RMSD bond angle	1.623
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.061	0.310
Total number of observations	281274 *
Number of reflections	26568 *	2687 *
<I/σ(I)>	20.3
Completeness [%]	99.8	100
Redundancy	10.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.5	277	PEG MME 5000, Phosphate, pH 6.5, VAPOR DIFFUSION/HANGING DROP, temperature 277K