1GFS
GDP-FUCOSE SYNTHETASE FROM E. COLI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 291 |
Detector technology | IMAGE PLATE |
Collection date | 1997-02 |
Detector | RIGAKU |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 104.200, 104.200, 74.900 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 14.000 - 2.200 |
R-factor | 0.171 |
Rwork | 0.171 |
R-free | 0.21400 |
Structure solution method | MIRAS |
RMSD bond length | 0.008 |
RMSD bond angle | 1.360 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 14.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.052 | |
Number of reflections | 23163 | |
<I/σ(I)> | 33.1 | 4.5 |
Completeness [%] | 95.9 | 75.8 |
Redundancy | 9.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.4 * | pH 7.00 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6 (mg/ml) | |
2 | 1 | drop | Tris | 10 (mM) | |
3 | 1 | drop | 50 (mM) | ||
4 | 1 | reservoir | sodium formate | 4.0 (M) |