1GEF

Crystal structure of the archaeal holliday junction resolvase HJC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL24XU
Synchrotron site	SPring-8
Beamline	BL24XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-05-23
Detector	RIGAKU RAXIS IV
Wavelength(s)	0.834
Spacegroup name	P 1 21 1
Unit cell lengths	36.245, 119.056, 63.146
Unit cell angles	90.00, 90.66, 90.00

Refinement procedure

Resolution	20.000 - 2.000
Rwork	0.224
R-free	0.26000 *
Structure solution method	MAD
RMSD bond length	0.005 *
RMSD bond angle	1.210 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.034	0.149
Number of reflections	29280
<I/σ(I)>	20
Completeness [%]	81.2 *	76.4
Redundancy	2.2	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	7	20 *	PEG 4000, ammonium sulfate, EDTA, glycerol, Tris-HCl, pH 7.0, VAPOR DIFFUSION, temperature 297K
1	Batch method *	7	20 *	PEG 4000, ammonium sulfate, EDTA, glycerol, Tris-HCl, pH 7.0, VAPOR DIFFUSION, temperature 297K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	Tris-HCl	100 (mM)
2	1	1	EDTA	1 (mM)
3	1	1	PEG4000	27.5 (%(w/v))
4	1	1	ammonium sulfate	50 (mM)
5	1	1	glycerol	10 (%)