1GCB

GAL6, YEAST BLEOMYCIN HYDROLASE DNA-BINDING PROTEASE (THIOL)

Experimental procedure

Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 63 2 2
Unit cell lengths	151.150, 151.150, 89.880
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.200
R-factor	0.209
Rwork	0.209
R-free	0.27400
RMSD bond length	0.008
RMSD bond angle	1.380
Data reduction software	MSC
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.200
Rmerge	0.074
Total number of observations	202481 *
Number of reflections	31451
Completeness [%]	99.8
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5 *	22 *
1	Vapor diffusion, hanging drop *	8.5 *	22 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	36 (mg/ml)
2	1	drop	Tris-HCl	25 (mM)
3	1	drop	glycerol	10 (%)
4	1	reservoir	PEG4000	21 (%)
5	1	reservoir	ammonium sulfate	0.3 (M)
6	1	reservoir	DTT	2 (mM)
7	1	reservoir	Tris-HCl	100 (mM)