1GCB
GAL6, YEAST BLEOMYCIN HYDROLASE DNA-BINDING PROTEASE (THIOL)
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 151.150, 151.150, 89.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.200 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.27400 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.380 |
| Data reduction software | MSC |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.074 |
| Total number of observations | 202481 * |
| Number of reflections | 31451 |
| Completeness [%] | 99.8 |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 * | 22 * | |
| 1 | Vapor diffusion, hanging drop * | 8.5 * | 22 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 36 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 25 (mM) | |
| 3 | 1 | drop | glycerol | 10 (%) | |
| 4 | 1 | reservoir | PEG4000 | 21 (%) | |
| 5 | 1 | reservoir | ammonium sulfate | 0.3 (M) | |
| 6 | 1 | reservoir | DTT | 2 (mM) | |
| 7 | 1 | reservoir | Tris-HCl | 100 (mM) |
