1GA5
CRYSTAL STRUCTURE OF THE ORPHAN NUCLEAR RECEPTOR REV-ERB(ALPHA) DNA-BINDING DOMAIN BOUND TO ITS COGNATE RESPONSE ELEMENT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 1997-12-15 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9054 |
Spacegroup name | P 1 |
Unit cell lengths | 44.920, 52.020, 78.880 |
Unit cell angles | 85.84, 76.61, 74.48 |
Refinement procedure
Resolution | 19.600 - 2.400 |
R-factor | 0.253 * |
Rwork | 0.253 |
R-free | 0.29900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a6y residues 132-198 from chain A & B plus DNA |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.600 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.083 | 0.323 * |
Number of reflections | 25370 | 2476 * |
<I/σ(I)> | 6.8 | 2.5 |
Completeness [%] | 87.3 * | 82.8 * |
Redundancy | 2.8 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 17 * | Zhao, Q., (1998) Mol. Cell, 1, 849. * |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG8000 | ||
2 | 1 | 1 | MgCl2 | ||
3 | 1 | 1 | NaCl | ||
4 | 1 | 1 | Tris |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | DNA | 0.6 (mM) | |
2 | 1 | drop | protein | 1.2 (mM) | |
3 | 1 | reservoir | PEG8000 | 20-25 (%) | |
4 | 1 | reservoir | 5 (mM) | ||
5 | 1 | reservoir | 400 (mM) | ||
6 | 1 | reservoir | Tris | pH7.5 |