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1G9T

CRYSTAL STRUCTURE OF E.COLI HPRT-GMP COMPLEX

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 14-BM-D
Synchrotron siteAPS
Beamline14-BM-D
Temperature [K]100
Detector technologyCCD
Collection date1999-12-20
DetectorADSC QUANTUM 1
Wavelength(s)1.00
Spacegroup nameP 31 2 1
Unit cell lengths84.140, 84.140, 167.270
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution50.000 - 2.800
R-factor0.199
Rwork0.199
R-free0.24400

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Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)T.foetus HPRT
RMSD bond length0.007
RMSD bond angle1.380

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Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareX-PLOR
Refinement softwareCNS (1.0)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.900
High resolution limit [Å]2.8002.800
Rmerge0.0720.294
Total number of observations45289

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Number of reflections15697

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<I/σ(I)>9.14.1
Completeness [%]88.989.8
Redundancy2.91.2
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.4

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20

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Hepes, sodium citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein18 (mg/ml)0.88mM in terms of subumits
21dropTris-HCl0.05 (M)
31drop0.01 (M)
41dropdithiothreitol1 (mM)pH7.4
51reservoirHEPES0.1 (M)pH7.5
61reservoirsodium citrate0.1 (M)

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PDB entries from 2024-03-27

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