1G85
CRYSTAL STRUCTURE OF BOVINE ODORANT BINDING PROTEIN COMPLEXED WITH IS NATURAL LIGAND
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-05-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.900, 65.500, 42.700 |
| Unit cell angles | 90.00, 98.80, 90.00 |
Refinement procedure
| Resolution | 25.300 - 1.800 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pbo |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.000 | 55.000 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.084 | 0.230 |
| Number of reflections | 107952 | |
| <I/σ(I)> | 5 | |
| Completeness [%] | 45.0 | 97.8 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 5.7 | 277 | 38% ethanol, 25mM sodium citrate, pH 5.7, MICRODIALYSIS, temperature 4K, temperature 277K |
