1G61
CRYSTAL STRUCTURE OF M.JANNASCHII EIF6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2000-02-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.91825 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.067, 46.964, 84.607 |
| Unit cell angles | 90.00, 98.53, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.300 |
| R-factor | 0.132 |
| Rwork | 0.132 |
| R-free | 0.17900 |
| Structure solution method | MAD |
| RMSD bond length | 0.019 * |
| RMSD bond angle | 2.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 25.000 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.036 | 0.235 |
| Total number of observations | 463351 * | |
| Number of reflections | 110636 | |
| <I/σ(I)> | 34 | 6.6 |
| Completeness [%] | 97.7 | 95.4 * |
| Redundancy | 4.2 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 4 * | 31% PEG-MME 2000, 0.200M Ammonium sulfate, 0.100M MES pH 5.6, 3mM Xylitol, 10mM glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG2000 MME | 31 (%(w/v)) | |
| 2 | 1 | reservoir | MES | 100 (mM) | pH5.6 |
| 3 | 1 | reservoir | ammonium sulfate | 200 (mM) | |
| 4 | 1 | reservoir | xylitol | 3 (mM) | |
| 5 | 1 | reservoir | glycerol | 10 (%(v/v)) | |
| 6 | 1 | reservoir | TCEP | 1 (mM) | |
| 7 | 1 | drop | protein | 3.5 (mg/ml) |
