1G50
CRYSTAL STRUCTURE OF A WILD TYPE HER ALPHA LBD AT 2.9 ANGSTROM RESOLUTION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 1997-04-01 |
Wavelength(s) | 0.9934 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 105.500, 105.500, 136.080 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 * - 2.900 |
R-factor | 0.241 |
Rwork | 0.241 |
R-free | 0.31000 |
Starting model (for MR) | 1qkt |
RMSD bond length | 0.014 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | |
High resolution limit [Å] | 2.900 | 2.900 * |
Rmerge | 0.080 * | |
Number of reflections | 18142 | |
Completeness [%] | 91.0 | 78 * |
Redundancy | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 * | 4 * | PEG400, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
10 | 1 | reservoir | PEG400 | 4-20 (%) | |
11 | 1 | reservoir | 350-950 (mM) | ||
12 | 1 | reservoir | imidazole | 50 (mM) | |
13 | 1 | reservoir | beta-mercaptoethanol | 50 (mM) | |
2 | 1 | drop | 50 (mM) | ||
3 | 1 | drop | Tris-maleate/HCl | 50 (mM) | pH8. |
4 | 1 | drop | estradiol | 0.010 (mM) | |
5 | 1 | drop | beta-mercaptoethanol | 50 (mM) | |
6 | 1 | drop | PEG400 | 2-10 (%) | |
7 | 1 | drop | 400 (mM) | ||
8 | 1 | drop | beta-mercaptoethanol | 50 (mM) | |
9 | 1 | drop | imidazole | 50 (mM) | pH7.3 |